Cytolytic leukotoxins of the repeat in toxin (RTX) family are large proteins excreted by gram-negative bacterial pathogens through the type 1 secretion system (T1SS). Due to low yields and poor stability in cultures of the original pathogens. it is useful to purify recombinant fatty-acylated RTX cytolysins from inclusion bodies produced in E. coli. https://www.littletreehouselanes.shop/product-category/open-play/